韩瑞枝

职称：教授，硕士生导师

学历/学位：研究生、博士

电话：

电子邮箱：hanrz@jiangnan.edu.cn

简介

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从事蛋白质工程，酶工程的研究，主要承担《酶工程》、《蛋白质工程实验》、《生物化学实验》课程的讲授工作。近年来共发表高水平研究论文十余篇，累计影响因子达50，他引200余次，申请发明专利40项，授权发明专利15项。主持包括国家自然科学基金、江苏省自然科学基金，“绿色生物制造”重点研发子课题，中国博士后基金等在内的省部级科研项目5项；获得教育部科技进步二等奖1项（2019,3/6）,江苏省优秀博士论文（2015）

学习工作经历

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2003-2007合肥工业大学，生物工程专业，工科学士

2007-2010四川大学，发酵工程专业，工科硕士

2010-2014江南大学，发酵工程专业，工科博士

2014-2024江南大学，副教授

2024-至今江南大学，教授

2022-2023德国亚琛工业大学，Ulrich Schwaneberg课题组，访问学者

主要成果

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一、论文（论著）发表情况

部分代表性论文如下：

1、Ruizhi Han, Ke Gao, Yulin Jiang, Jieyu Zhou, Guochao Xu, Jinjun Dong, Ulrich Schwaneberg, Yu Ji, Ye Ni. (2023) Self-Sufficient In Vitro Multi-Enzyme Cascade for Efficient Synthesis of Danshensu from l-DOPA, ACS Synthetic Biology (封面文章), , 12(1): 277-286

2、Ruizhi Han, Honghui Fang, Zhaoyue Fan, et al. (2023) Coupled reaction of glycosyltransferase and sucrose synthase for high-yielding and cost-effective synthesis of rosin. Molecular Catalysis, 540: 113035

3、Han, R., Chai, B., Jiang, Y., Ni, J., & Ni, Y. (2022). Engineering of cyclodextrin glycosyltransferase from Paenibacillus macerans for enhanced product specificity of long-chain glycosylated sophoricosides. Molecular Catalysis, 519, 112147.

4、Han, R., Cao, X., Fang, H., Zhou, J., & Ni, Y. (2021). Structure-based engineering of ω-transaminase for enhanced catalytic efficiency toward (R)-(+)-1-(1-naphthyl) ethylamine synthesis. Molecular Catalysis, 502, 111368.

5、Rui-Zhi Han, Jie Ni, Jieyu Zhou, et al. (2020) Engineering of cyclodextrin glycosyltransferase reveals pH-regulatory mechanism of enhanced long chain glycosylated sophoricoside specificity. Applied and Environmental Microbiology, 86(7), e00004-20

6、Dong Yao, Jia Fan, Ruizhi Han*, et al.(2020) Enhancing soluble expression of sucrose phosphorylase in Escherichia coli by molecular chaperones. Protein Expression and Purification, 169, 105571

7、Ruizhi Han, Binbin Ge, Ming Yang, et al. (2017) High production of genistein diglucoside derivative using cyclodextrin glycosyltransferase from Paenibacillus macerans. Journal of Industrial Microbiology & Biotechnology, 44, 1343-1354

8、Rui-Zhi Han, Guo-Chao Xu, Jin-Jun Dong, Ye Ni*. Arginine deiminase: recent advances in discovery, crystal structure and protein engineering for improved properties as an anti-tumor drug. Applied Microbiology & Biotechnology (2016) 100: 4747-4760

9、Han, R.Z., Li, J.H., Shin, H.D. et al. (2014) Recent advances in discovery, heterologous expression, and molecular engineering of cyclodextrin glycosyltransferase for versatile applications. Biotechnology Advances, 32, 415-428.

10、Han, R.Z., Li, J.H., Shin, H.D. et al. (2014) Fusion of self-assembling amphipathic oligopeptides with cyclodextrin glycosyltransferase improves 2-O-D-glucopyranosyl-l-ascorbic acid synthesis with soluble starch as the glycosyl donor. Applied and Environmental Microbiology, 80, 4717-4724.

11、Han, R.Z., Liu, L., Shin, H.D. et al. (2013) Iterative saturation mutagenesis of -6 subsite residues in cyclodextrin glycosyltransferase from Paenibacillus macerans to improve maltodextrin specificity for 2-Od-glucopyranosyl-l-ascorbic acid synthesis. Applied and Environmental Microbiology, 79, 7562-7568.

12、Han, R.Z., Li, J.H., Shin, H.D. et al. (2013) Carbohydrate-binding module-cyclodextrin glycosyltransferase fusion enables efficient synthesis of 2-O-d-glucopyranosyl-l-ascorbic acid with soluble starch as the glycosyl donor. Applied and Environmental Microbiology, 79, 3234-3240.

13、Han, R.Z., Liu, L., Shin, H.D. et al. (2013) Systems engineering of tyrosine 195, tyrosine 260, and glutamine 265 in cyclodextrin glycosyltransferase from Paenibacillus macerans to enhance maltodextrin specificity for 2-O-(D)-glucopyranosyl-(L)-ascorbic acid synthesis. Applied and Environmental Microbiology, 79, 672-677.

14、Han, R.Z., Liu, L., Shin, H.D. et al. (2013) Site-saturation engineering of lysine 47 in cyclodextrin glycosyltransferase from Paenibacillus macerans to enhance substrate specificity towards maltodextrin for enzymatic synthesis of 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G). Applied Microbiology and Biotechnology, 97, 5851-5860.

15、Han, R.Z., Liu, L., Li, J.H. et al. (2012) Functions, applications and production of 2-O-Dglucopyranosyl-L-ascorbic acid. Applied Microbiology and Biotechnology, 95, 313-320.

二、专利情况

1.Method for producing long-chain glycosylated genistein. US Patent No.: US 11203743B2. Ruizhi Han, Ye Ni, Baocheng Chai. Date of Patent: 12.21.2021

2.一种利用环糊精葡萄糖基转移酶高产染料木素糖基化衍生物的方法.专利号ZL2017100149560,申请人：江南大学.发明人：韩瑞枝，倪晔，姜明杨，葛彬彬，许国超，董晋军

3.一种环糊精葡萄糖基转移酶及其制备方法专利号：ZL201810303894X，申请人：江南大学.发明人：韩瑞枝，倪晔，葛彬彬，姚栋，董进军，许国超，

4.一种分子伴侣共表达提高蔗糖磷酸化酶表达效率的方法授权号：ZL2018114159083申请人：江南大学，山东隆科特酶制剂有限公司.发明人：韩瑞枝，姚栋，倪晔，肖静，王克芬

5.一种产物特异性提高的环糊精葡萄糖基转移酶及制备方法.授权号：ZL2018102995436,申请人：江南大学.发明人：韩瑞枝,倪晔,葛彬彬,姚栋,董晋军,许国超.

6.一种生蔗糖磷酸化酶的方法及应用.授权号：ZL2019110803754,申请人：江南大学，山东隆科特酶制剂有限公司.发明人：韩瑞枝，姚栋，胡云箫，倪晔，肖静，王克芬

7.一种环糊精葡萄糖基转移酶突变体及其应用.授权号：ZL2019110847002,申请人：江南大学.发明人：韩瑞枝，柴宝成，倪晔

8.一种生产长链糖基化染料木素的方法.授权号：ZL2019110847695,申请人：江南大学.发明人：韩瑞枝，柴宝成，潘艳莹，倪晔

三、承担教学科研项目情况

1、国家自然科学基金面上基金，31871738，研究期限：2019.1-2022.12；

2、“绿色生物制造”国家重点研发项目子课题，2021YFC2102700，研究期限：2021.1-2024.12

3、国家自然科学基金青年基金，31401634，研究期限：2015.1-2017.12（已结题）；

4、江苏省自然科学基金-青年基金，BK20140135，研究期限：2014.7-2017.6（已结题）；

5、中国博士后基金第62批面上基金，2017M621631，研究期限：2017.11-2020.8（已结题）

四、获奖情况（含指导学生获奖）

1、江苏省优秀博士论文，2015，（1/1）

2、教育部科技进步奖二等奖，2019，（3/6）

3、中国石油与化学工业联合会科技进步二等奖（3/6）

以上资料更新时间截止：2023年4月