一、个人简历
丁学峰，男，1990年09月出生，博士、博士后。
主要经历
2011.09—2015.06烟台大学，生命科学学院，学士
2016.09—2023.06江南大学，生物工程学院，博士
2023.07—至今江南大学，生命科学与健康工程学院，博士后
二、研究领域
（1）CHO细胞和293细胞稳定表达位点的寻找及鉴定
（2）治疗性药用蛋白的绿色生物制造
（3）CHO细胞和293细胞工程化改造
三、学术成果
（一）近5年来代表性论文
1、Yongqiang Deng,Xuefeng Ding（共一）, Qiyue Wu, Yanfei Cai, Jian Jin, Qinghe Xu, Jingyu Zhu, Yun Chen, (2023). Construction of a stable expression HEK293T engineered cell line adapted to high-density suspension culture,Biochemical Engineering Journal，Volume 207,2024,109334,ISSN 1369-703X.
2、Ding X,Chen Y, Yang Z, Wu H, Cai Y, Wang E, Jin J, Li H. (2023). A comprehensive evaluation of stable expression "hot spot" in the ScltI gene of Chinese hamster ovary cells.Appl Microbiol Biotechnol.107(4):1299-1309. doi: 10.1007/s00253-023-12383-w.
3、Hao Wu,Xuefeng Ding, Yun Chen, Yanfei Cai, Zhaoqi Yang, Jian Jin, (2023). EGFR-targeted humanized single chain antibody fragment functionalized silica nanoparticles for precision therapy of cancer,International Journal of Biological Macromolecules, Volume 253, Part 8,2023,127538, ISSN 0141-8130,
4、Hao Wu,Xuefeng Ding, Yun Chen, Yanfei Cai, Zhaoqi Yang, and Jian Jin. (2023). Constructed Tumor-Targeted and MMP-2 Biocleavable Antibody Conjugated Silica Nanoparticles for Efficient Cancer Therapy.nACS Omega, 8 (14), 12752-12760 DOI: 10.1021/acsomega.2c07949
5、Hao Wu, Xuefeng Ding, Yun Chen, Yanfei Cai, Zhaoqi Yang, Qinghe Xu, Jian Jin, (2023). Analysis of the in vitro function and internalization ability of a humanized EGFR antibody AE01 expressed by Chinese hamster ovary cells,Protein Expression and Purification, Volume 206, 106243, ISSN 1046-5928,https://doi.org/10.1016/j.pep.2023.106243.
6、Ding, XF;Chen, Y;Wu, H;Yang, ZQ;Cai, YF;Dai, YF;Xu, QH;Jin, J;Li, HZ,(2022).Construction of a CHO cell line with site-specific integration to stably express exogenous proteins using the CRISPR-Cas9 technique,SYSTEMS MICROBIOLOGY AND BIOMANUFACTURING,Volume3, Issue4,Page659-668.
7、Zhou, S.,Ding, X., Yang, L., Chen, Y., Gong, X., Jin, J., & Li, H. (2019). Discovery of a stable expression hot spot in the genome of Chinese hamster ovary cells using lentivirus-based random integration.Biotechnology & Biotechnological Equipment, 33(1), 605–612.https://doi.org/10.1080/13102818.2019.1601998
（二）近5年主要授权专利
1、2022.07，发明专利，一种CHO细胞基因NW_003614092.1内稳定表达蛋白质的位点及其应用，专利号：ZL202111395539.8
2、2022.07，发明专利，一种CHO细胞基因NW_003613781.1内稳定表达蛋白质的位点及其应用，专利号：ZL202111415460.7
3、2022.07，发明专利，一种CHO细胞基因NW_003614889.1内稳定表达蛋白质的位点及其应用，专利号：ZL202111395511.4
4、2022.07，发明专利，一种CHO细胞基因NW_003613756.1内稳定表达蛋白质的位点及其应用，专利号：ZL202111395490.6
四、联系方式
通讯地址：江苏省无锡市蠡湖大道1800号江南大学生命科学与健康工程学院
邮编：214122
邮箱：8202307003@jiangnan.edu.cn
